Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ssDNA | the RadA protein is a ssDNA-dependent ATPase | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Saccharolobus solfataricus | the RadA protein is a DNA-dependent ATPase, forms a nucleoprotein filament on DNA, and catalyzes DNA pairing and strand exchange | ADP + phosphate | - |
? | |
ATP + H2O | Saccharolobus solfataricus P2 | the RadA protein is a DNA-dependent ATPase, forms a nucleoprotein filament on DNA, and catalyzes DNA pairing and strand exchange | ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q55075 | - |
- |
Saccharolobus solfataricus P2 | Q55075 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | the RadA protein is a DNA-dependent ATPase, forms a nucleoprotein filament on DNA, and catalyzes DNA pairing and strand exchange | Saccharolobus solfataricus | ADP + phosphate | - |
? | |
ATP + H2O | the RadA protein is a ssDNA-dependent ATPase. ATP hydrolysis is less efficient in the presence of double-stranded DNA, and almost no hydrolysis occurs in the absence of DNA. It forms a nucleoprotein filament on DNA, and catalyzes DNA pairing and strand exchange | Saccharolobus solfataricus | ADP + phosphate | - |
? | |
ATP + H2O | the RadA protein is a DNA-dependent ATPase, forms a nucleoprotein filament on DNA, and catalyzes DNA pairing and strand exchange | Saccharolobus solfataricus P2 | ADP + phosphate | - |
? | |
ATP + H2O | the RadA protein is a ssDNA-dependent ATPase. ATP hydrolysis is less efficient in the presence of double-stranded DNA, and almost no hydrolysis occurs in the absence of DNA. It forms a nucleoprotein filament on DNA, and catalyzes DNA pairing and strand exchange | Saccharolobus solfataricus P2 | ADP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RadA | - |
Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 85 | DNA-dependent ATP hydrolysis occurrs exclusively at elevated temperatures (65°C, 75°C, 85°C), and very little hydrolysis occurrs at 37°C | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
ATP | pH 7.5, 37°C, 6 mM MgCl2, addition of poly(dT) | Saccharolobus solfataricus | |
0.12 | - |
ATP | pH 7.5, 65°C, 6 mM MgCl2, addition of poly(dT) | Saccharolobus solfataricus | |
0.14 | - |
ATP | pH 7.5, 75°C, 6 mM MgCl2, addition of poly(dT) | Saccharolobus solfataricus | |
0.17 | - |
ATP | pH 7.5, 85°C, 6 mM MgCl2, addition of poly(dT) | Saccharolobus solfataricus |